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6 gram EAA har stimulerar proteinsyntes i princip dubbelt så mycket som 3 gram EAA.
40 gram EAA stimulerar inte proteinsyntes mer än 20 gram EAA.
Under 2-timmarsperioden följande träning krävs maximalt (vikt i kg * 0.260 mg/kg/h) protein för att stimulera maximalt anabolt gensvar, dvs 40 g protein dessa 2 timmar för en 80-kilosperson. Tar man 20 gram i timmen, med början direkt efter träning, blir det 20+20+20 = tillräckligt för den störste.
Tipton, K.D., Ferrando, A.A., Phillips, S.M., Doyle, D., Jr.
and Wolfe, R.R. (1999). Postexercise net protein synthesis
in human muscle from orally administered amino acids.
American Journal of Physiology, 276, E628–E634.
Stuart M. Phillips, PhD, Protein Requirements and Supplementation in
Strength Sports, Nutrition 2004;20:689–695.
"there may be a point of essential amino acid availability above
which no further stimulation occurs. Support for this
concept comes from the fact that net muscle protein
synthesis was similar when 20 g and 40 g of essential
amino acids were ingested after resistance exercise.
Any further stimulation must occur following the next meal."
Tipton KD, Wolfe RR., Protein and amino acids for athletes, J Sports Sci. 2004 Jan;22(1):65-79.
"it appears that only modest amounts of dietary amino acids would be needed to achieve maximal stimulation of the muscle anabolic processes i.e. for adults of average weight, 55-75 kg × 0.260 mg/kg/h × 2 h, or of the order of 30-40 g of protein."
Bohe J, Low A, Wolfe RR, Rennie MJ, Human muscle protein synthesis is modulated by extracellular not intramuscular amino acid availability: a dose response study, J Physiol. 2003 Oct 1;552(Pt 1):315-24.