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This method is the simplified modification of the AACC total dietary
fiber (TDF) method, 32-05.01, and the AACC soluble/insoluble dietary
fiber method (for oat products), 32-21.01. (See Note 1, page 9.)
1. Principle:
Briefly, 1 g dried food samples (duplicate) is subjected to
sequential enzymatic digestion by heat-stable α-amylase, protease and
amyloglucosidase.
2. Soluble/insoluble dietary fibre determination:
Insoluble dietary fibre (IDF) is filtered, and then residue is washed with warm
distilled water. Combined solution of filtrate and water washings are
precipitated with 4 volumes of 95% ethanol (EtOH) for soluble dietary
fibre (SDF) determination. Precipitate is then filtered and dried. Both
SDF and IDF residues are corrected for protein, ash and blank, for the
final calculation of SDF and IDF values.
3. Total dietary fibre determination:
SDF is precipitated with EtOH, and residue is then filtered, dried and weighed. Total dietary fibre (TDF) value is corrected for protein and ash content.
SCOPE:
This method determines soluble, insoluble and total dietary fibre
content in processed foods and raw materials, such as cereal products,
fruits and vegetables.