King Grub
2010-06-11, 09:44
Context: GH secretion is increased by stress, such as fasting and exercise.
Objective: Our study was designed to test the hypothesis that fasting and exercise increase GH levels, GH-dependent signal transducer and activator of transcription (STAT)-5b phosphorylation, and IGF-I mRNA levels in human skeletal muscle.
Design and Participants: Eight healthy men (25.5 +/- 4.3 yr) were studied for 6 h (a 4 h basal and a 2 h clamp period) in the basal state and after 72 h fasting and a 1-h ergometer cycling at 65% maximal oxygen uptake. Muscle biopsies were taken at t = 30 and 270 min.
Setting: The study was conducted at a university clinical research unit. Results: During exercise, GH concentrations rapidly increased to greater than 10 ng/ml (P < 0.001). Thirty minutes after exercise, we observed a 4-fold increase in signal transducer and activator of transcription (STAT)-5 phosphorylation (P < 0.001) followed by an increase in IGF-I mRNA after 270 min (P = 0.026). During fasting, more sporadic GH bursts occurred, leading to an overall 3-fold increase in GH area under the curvet=0-270 (P < 0.001). Similarly, STAT5 patterns were less consistent, with a tendency toward increased phosphorylation (P = 0.067, area under the curvet=0-270), whereas IGF-I mRNAs were persistently increased (P < 0.01).
Conclusions: Our data show that myocellular GH signaling is stimulated after exercise and fasting in terms of increased STAT5 phosphorylation and/or IGF-I gene expression. This suggests that exercise with brief, well-defined GH peaks leads to distinct STAT5 phosphorylation and subsequent IGF-I gene expression, whereas fasting induces more sporadic GH bursts and less distinct but more persistent activation of the GH signal.
J Clin Endocrinol Metab. 2010 Jun 9. Exercise and Fasting Activate Growth Hormone-Dependent Myocellular Signal Transducer and Activator of Transcription-5b Phosphorylation and Insulin-Like Growth Factor-I Messenger Ribonucleic Acid Expression in Humans.
http://jcem.endojournals.org/cgi/rapidpdf/jc.2010-0689v1
Objective: Our study was designed to test the hypothesis that fasting and exercise increase GH levels, GH-dependent signal transducer and activator of transcription (STAT)-5b phosphorylation, and IGF-I mRNA levels in human skeletal muscle.
Design and Participants: Eight healthy men (25.5 +/- 4.3 yr) were studied for 6 h (a 4 h basal and a 2 h clamp period) in the basal state and after 72 h fasting and a 1-h ergometer cycling at 65% maximal oxygen uptake. Muscle biopsies were taken at t = 30 and 270 min.
Setting: The study was conducted at a university clinical research unit. Results: During exercise, GH concentrations rapidly increased to greater than 10 ng/ml (P < 0.001). Thirty minutes after exercise, we observed a 4-fold increase in signal transducer and activator of transcription (STAT)-5 phosphorylation (P < 0.001) followed by an increase in IGF-I mRNA after 270 min (P = 0.026). During fasting, more sporadic GH bursts occurred, leading to an overall 3-fold increase in GH area under the curvet=0-270 (P < 0.001). Similarly, STAT5 patterns were less consistent, with a tendency toward increased phosphorylation (P = 0.067, area under the curvet=0-270), whereas IGF-I mRNAs were persistently increased (P < 0.01).
Conclusions: Our data show that myocellular GH signaling is stimulated after exercise and fasting in terms of increased STAT5 phosphorylation and/or IGF-I gene expression. This suggests that exercise with brief, well-defined GH peaks leads to distinct STAT5 phosphorylation and subsequent IGF-I gene expression, whereas fasting induces more sporadic GH bursts and less distinct but more persistent activation of the GH signal.
J Clin Endocrinol Metab. 2010 Jun 9. Exercise and Fasting Activate Growth Hormone-Dependent Myocellular Signal Transducer and Activator of Transcription-5b Phosphorylation and Insulin-Like Growth Factor-I Messenger Ribonucleic Acid Expression in Humans.
http://jcem.endojournals.org/cgi/rapidpdf/jc.2010-0689v1