z_bumbi
2005-05-02, 09:09
The influence of n-3 polyunsaturated fatty acids and very low calorie diet (VLCD) during a short-term weight reducing regimen on weight loss and serum fatty acid composition in severely obese women.
Kunesova M, Braunerova R, Hlavaty P, Tvrzicka E, Stankova B, Skrha J, Hilgertova J, Hill M, Kopecky J, Wagenknecht M, Hainer V, Matoulek M, Parizkova J, Zak A, Svacina S.
Obesity Management Centre, Institute of Endocrinology, Narodni 8, 116 94 Prague 1, Czech Republic mkunesova@endo.cz, marie.kunesova@lf1.cuni.cz.
Polyunsaturated fatty acids of n-3 series (n-3 PUFA) were shown to increase basal fat oxidation in humans. The aim of the study was to compare the effect of n-3 PUFA added to a very low calorie diet (VLCD), with VLCD only during three-week inpatient weight reduction. 20 severely obese women were randomly assigned to VLCD with n-3 PUFA or with placebo. Fatty acids in serum lipid fractions were quantified by gas chromatography. Differences between the groups were determined using ANOVA. Higher weight (7.55+/-1.77 vs 6.07+/-2.16 kg, NS), BMI (2.82+/-0.62 vs 2.22+/-0.74, p<0.05) and hip circumference (7.8+/-9.37 vs 2.5+/-2.5cm, p<0.05) losses were found in the n-3 group as compared to the control group. Significantly higher increase in beta-hydroxybutyrate was found in the n-3 group showing higher ketogenesis and possible higher fatty acid oxidation. Increase in beta-hydroxybutyrate significantly correlated with increase in serum phospholipid arachidonic acid (20:4n-6; r = 0.91, p<0.001). In the n-3 group significantly higher increase was found in n-3 PUFA (eicosapentaenoic acid, 20:5n-3, docosahexaenoic acid, 22:6n-3) in trigycerides and phospholipids; in triglycerides significant decrease in palmitoleic acid (16:1n-7) and in vaccenic acid (18:1n-7) probably reflected lower lipogenesis. A significant negative correlation between BMI change and phospholipid docosahexaenic acid change was found (r = -0.595, p<0.008). The results suggest that long chain n-3 PUFA enhance weight loss in obese females treated by VLCD. Docosahexaenoate (22:6n-3) seems to be the active component.
Kunesova M, Braunerova R, Hlavaty P, Tvrzicka E, Stankova B, Skrha J, Hilgertova J, Hill M, Kopecky J, Wagenknecht M, Hainer V, Matoulek M, Parizkova J, Zak A, Svacina S.
Obesity Management Centre, Institute of Endocrinology, Narodni 8, 116 94 Prague 1, Czech Republic mkunesova@endo.cz, marie.kunesova@lf1.cuni.cz.
Polyunsaturated fatty acids of n-3 series (n-3 PUFA) were shown to increase basal fat oxidation in humans. The aim of the study was to compare the effect of n-3 PUFA added to a very low calorie diet (VLCD), with VLCD only during three-week inpatient weight reduction. 20 severely obese women were randomly assigned to VLCD with n-3 PUFA or with placebo. Fatty acids in serum lipid fractions were quantified by gas chromatography. Differences between the groups were determined using ANOVA. Higher weight (7.55+/-1.77 vs 6.07+/-2.16 kg, NS), BMI (2.82+/-0.62 vs 2.22+/-0.74, p<0.05) and hip circumference (7.8+/-9.37 vs 2.5+/-2.5cm, p<0.05) losses were found in the n-3 group as compared to the control group. Significantly higher increase in beta-hydroxybutyrate was found in the n-3 group showing higher ketogenesis and possible higher fatty acid oxidation. Increase in beta-hydroxybutyrate significantly correlated with increase in serum phospholipid arachidonic acid (20:4n-6; r = 0.91, p<0.001). In the n-3 group significantly higher increase was found in n-3 PUFA (eicosapentaenoic acid, 20:5n-3, docosahexaenoic acid, 22:6n-3) in trigycerides and phospholipids; in triglycerides significant decrease in palmitoleic acid (16:1n-7) and in vaccenic acid (18:1n-7) probably reflected lower lipogenesis. A significant negative correlation between BMI change and phospholipid docosahexaenic acid change was found (r = -0.595, p<0.008). The results suggest that long chain n-3 PUFA enhance weight loss in obese females treated by VLCD. Docosahexaenoate (22:6n-3) seems to be the active component.