King Grub
2020-04-16, 08:15
SCOPE:
Low-calorie sweetener (LCS) consumption is associated with metabolic disease in observational studies. However, physiologic mechanisms underlying LCS-induced metabolic impairments in humans are unclear. This study aimed to identify molecular pathways in adipose impacted by LCS consumption.
METHODS AND RESULTS:
Seven females with overweight or obesity, who did not report LCS use, consumed 12-ounces of diet soda containing sucralose and acesulfame-potassium (Ace-K) three times daily for 8-weeks. A subcutaneous adipose biopsy from the left abdomen and fasting blood sample were collected at baseline and post-intervention. Global gene expression changes were assessed using RNA-sequencing followed by functional pathway analysis. No differences in circulating metabolic or inflammatory biomarkers were observed. However, ANOVA detected 828 differentially expressed annotated genes after diet soda consumption (p<0.05), including transcripts for inflammatory cytokines including interleukins and tumor-necrosis-family members. Fifty-eight of 140 canonical pathways represented in pathway analyses regulate inflammation, and several key upstream regulators of inflammation (e.g. TNF-alpha) were also represented.
CONCLUSION:
Consumption of diet soda with sucralose and Ace-K altered inflammatory transcriptomic pathways (e.g. NF-κB signaling) in subcutaneous adipose tissue but did not significantly alter circulating biomarkers. These findings highlight the need to examine molecular and metabolic effects of LCS exposure in a larger randomized control trial for a longer duration.
Mol Nutr Food Res. 2020 Apr 13:e1901166. Consumption of Diet Soda Sweetened with Sucralose and Acesulfame-Potassium Alters Inflammatory Transcriptome Pathways in Females with Overweight and Obesity.
Low-calorie sweetener (LCS) consumption is associated with metabolic disease in observational studies. However, physiologic mechanisms underlying LCS-induced metabolic impairments in humans are unclear. This study aimed to identify molecular pathways in adipose impacted by LCS consumption.
METHODS AND RESULTS:
Seven females with overweight or obesity, who did not report LCS use, consumed 12-ounces of diet soda containing sucralose and acesulfame-potassium (Ace-K) three times daily for 8-weeks. A subcutaneous adipose biopsy from the left abdomen and fasting blood sample were collected at baseline and post-intervention. Global gene expression changes were assessed using RNA-sequencing followed by functional pathway analysis. No differences in circulating metabolic or inflammatory biomarkers were observed. However, ANOVA detected 828 differentially expressed annotated genes after diet soda consumption (p<0.05), including transcripts for inflammatory cytokines including interleukins and tumor-necrosis-family members. Fifty-eight of 140 canonical pathways represented in pathway analyses regulate inflammation, and several key upstream regulators of inflammation (e.g. TNF-alpha) were also represented.
CONCLUSION:
Consumption of diet soda with sucralose and Ace-K altered inflammatory transcriptomic pathways (e.g. NF-κB signaling) in subcutaneous adipose tissue but did not significantly alter circulating biomarkers. These findings highlight the need to examine molecular and metabolic effects of LCS exposure in a larger randomized control trial for a longer duration.
Mol Nutr Food Res. 2020 Apr 13:e1901166. Consumption of Diet Soda Sweetened with Sucralose and Acesulfame-Potassium Alters Inflammatory Transcriptome Pathways in Females with Overweight and Obesity.