King Grub
2016-11-08, 14:10
Context:
The effects of caloric restriction (CR) on in vivo muscle mitochondrial function in humans are controversial.
Objective:
To evaluate muscle mitochondrial function and associated transcriptional profiles in non-obese humans following 12 months of CR.
Design:
Individuals from an ancillary study of the CALERIE 2 randomized, controlled trial were assessed at Baseline and 12 months after a 25% CR or ad libitum (Control) diet.
Setting:
The study was performed at Pennington Biomedical Research Center in Baton Rouge, Louisiana, USA.
Participants:
51 (34 females, 25-50 years of age) healthy non-obese individuals randomized to one of two groups (CR or Control).
Intervention:
12 months of a 25% CR or ad libitum (Control) diet.
Main Outcome(s):
In vivo mitochondrial function (ATPmax, P/O) by 31P-MRS and optical spectroscopy and body composition by DXA. In a subset of individuals, a muscle biopsy was performed for transcriptional profiling via qRT-PCR and microarrays.
Results:
Weight, BMI, fat and fat-free mass (p<0.001 for all) significantly decreased at Month 12 following CR vs. Control. In vivo ATPmax and P/O were unaffected by 12 months of CR. Targeted transcriptional profiling showed no effects on pathways involved in mitochondrial biogenesis, function or oxidative stress. A subgroup analysis according to Baseline P/O demonstrated that a higher (vs. lower) P/O was associated with significant improvements in ATPmax and P/O following CR.
Conclusions:
In the context of healthy non-obese humans, CR has no effect on muscle mitochondrial function, yet having a ‘more coupled’ (vs. ‘less coupled’) phenotype enables CR-induced improvements in muscle mitochondrial function.
Effects of 12 Months of Caloric Restriction on Muscle Mitochondrial Function in Healthy Individuals. JCEM, 25 October 2016.
http://press.endocrine.org/doi/pdf/10.1210/jc.2016-3211
The effects of caloric restriction (CR) on in vivo muscle mitochondrial function in humans are controversial.
Objective:
To evaluate muscle mitochondrial function and associated transcriptional profiles in non-obese humans following 12 months of CR.
Design:
Individuals from an ancillary study of the CALERIE 2 randomized, controlled trial were assessed at Baseline and 12 months after a 25% CR or ad libitum (Control) diet.
Setting:
The study was performed at Pennington Biomedical Research Center in Baton Rouge, Louisiana, USA.
Participants:
51 (34 females, 25-50 years of age) healthy non-obese individuals randomized to one of two groups (CR or Control).
Intervention:
12 months of a 25% CR or ad libitum (Control) diet.
Main Outcome(s):
In vivo mitochondrial function (ATPmax, P/O) by 31P-MRS and optical spectroscopy and body composition by DXA. In a subset of individuals, a muscle biopsy was performed for transcriptional profiling via qRT-PCR and microarrays.
Results:
Weight, BMI, fat and fat-free mass (p<0.001 for all) significantly decreased at Month 12 following CR vs. Control. In vivo ATPmax and P/O were unaffected by 12 months of CR. Targeted transcriptional profiling showed no effects on pathways involved in mitochondrial biogenesis, function or oxidative stress. A subgroup analysis according to Baseline P/O demonstrated that a higher (vs. lower) P/O was associated with significant improvements in ATPmax and P/O following CR.
Conclusions:
In the context of healthy non-obese humans, CR has no effect on muscle mitochondrial function, yet having a ‘more coupled’ (vs. ‘less coupled’) phenotype enables CR-induced improvements in muscle mitochondrial function.
Effects of 12 Months of Caloric Restriction on Muscle Mitochondrial Function in Healthy Individuals. JCEM, 25 October 2016.
http://press.endocrine.org/doi/pdf/10.1210/jc.2016-3211