tjing
2013-12-12, 15:45
The effects of oral glutamine supplementation on exercise induced gastrointestinal permeability and tight junction protein expression.
Abstract:
The purposes of this study are to assess whether 7 days of oral glutamine supplementation: (1) reduces exercise induced intestinal permeability; (2) prevents the pro-inflammatory response; and (3) to determine whether these changes are associated with the up-regulation of the heat shock response.
On separate occasions, eight human subjects participated in baseline testing, glutamine (GLN), and placebo (PLA) trials followed by a 60-min treadmill run. Intestinal permeability was higher in the PLA trial compared to baseline and GLN (0.0604 ± 0.047 vs. 0.0218 ±0.008 and 0.0272 ± 0.007, respectively, p<0.05). PBMC IκBα expression was higher 240-min post-ex in GLN trial compared to PLA (1.411 ± 0.523 vs. 0.9839 ± 0.343, p<0.05). In vitro (Caco-2) we measured effects of glutamine supplementation (0 mM, 4 mM, and 6 mM) on heat-induced (37° or 41.8°C) HSP70, HSF-1, and occludin expression. HSF-1 and HSP70 levels increased in 6 mM 41ºC compared to 0 mM 41ºC (1.785 ± 0.495 vs. 0.6681 ± 0.290, and 1.973 ± 0.325 vs. 1.133 ± 0.129, respectively, p<0.05). Occludin levels increased after 4 mM 41ºC and 6 mM 41ºC compared to 0 mM 41ºC (1.236 ± 0.219 and 1.849 ± 0.564 vs. 0.7434 ± 0.027, p<0.001, respectively).
Glutamine supplementation prevented exercise-induced permeability, possibly through HSF-1 activation.
J Appl Physiol (1985). 2013 Nov 27.
http://www.ncbi.nlm.nih.gov/pubmed/24285149
Abstract:
The purposes of this study are to assess whether 7 days of oral glutamine supplementation: (1) reduces exercise induced intestinal permeability; (2) prevents the pro-inflammatory response; and (3) to determine whether these changes are associated with the up-regulation of the heat shock response.
On separate occasions, eight human subjects participated in baseline testing, glutamine (GLN), and placebo (PLA) trials followed by a 60-min treadmill run. Intestinal permeability was higher in the PLA trial compared to baseline and GLN (0.0604 ± 0.047 vs. 0.0218 ±0.008 and 0.0272 ± 0.007, respectively, p<0.05). PBMC IκBα expression was higher 240-min post-ex in GLN trial compared to PLA (1.411 ± 0.523 vs. 0.9839 ± 0.343, p<0.05). In vitro (Caco-2) we measured effects of glutamine supplementation (0 mM, 4 mM, and 6 mM) on heat-induced (37° or 41.8°C) HSP70, HSF-1, and occludin expression. HSF-1 and HSP70 levels increased in 6 mM 41ºC compared to 0 mM 41ºC (1.785 ± 0.495 vs. 0.6681 ± 0.290, and 1.973 ± 0.325 vs. 1.133 ± 0.129, respectively, p<0.05). Occludin levels increased after 4 mM 41ºC and 6 mM 41ºC compared to 0 mM 41ºC (1.236 ± 0.219 and 1.849 ± 0.564 vs. 0.7434 ± 0.027, p<0.001, respectively).
Glutamine supplementation prevented exercise-induced permeability, possibly through HSF-1 activation.
J Appl Physiol (1985). 2013 Nov 27.
http://www.ncbi.nlm.nih.gov/pubmed/24285149