King Grub
2012-08-15, 09:50
BACKGROUND & AIMS:
Amino acid transporters have been proposed as regulators of protein synthesis. The primary aim of this study was to determine whether amino acid transporter expression is increased in human muscle following resistance exercise (RE) coupled with essential amino acid (EAA) ingestion, and whether a differential response occurs with aging. Secondly, we aimed to compare this response to a previous study examining RE alone.
METHODS:
Young (n = 7, 30 ± 2 yr) and older men (n = 6, 70 ± 2 yr) ingested EAA 1 h after RE. Muscle biopsies were obtained at rest and 3 and 6 h post exercise to examine amino acid transporter mRNA and protein expression.
RESULTS:
In both age groups, RE + EAA increased mRNA of L-type amino acid transporter 1 (LAT1)/solute linked carrier (SLC)7A5, sodium-coupled neutral amino acid transporter 2 (SNAT2)/SLC38A2, and cationic amino acid transporter 1/SLC7A1 (p < 0.05). SNAT2 protein increased in young at 3 and 6 h (p < 0.05), whereas old maintained higher LAT1 protein (p < 0.05). Compared to RE alone, RE + EAA enhanced amino acid transporter expression only in young (p < 0.05).
CONCLUSIONS:
RE increases muscle amino acid transporter expression in young and older adults, however, post exercise EAA ingestion enhances amino acid transporter expression only in young indicating that aging may influence the function of specific amino acid transporters.
Clin Nutr. 2012 Aug 1. Aging differentially affects human skeletal muscle amino acid transporter expression when essential amino acids are ingested after exercise.
Amino acid transporters have been proposed as regulators of protein synthesis. The primary aim of this study was to determine whether amino acid transporter expression is increased in human muscle following resistance exercise (RE) coupled with essential amino acid (EAA) ingestion, and whether a differential response occurs with aging. Secondly, we aimed to compare this response to a previous study examining RE alone.
METHODS:
Young (n = 7, 30 ± 2 yr) and older men (n = 6, 70 ± 2 yr) ingested EAA 1 h after RE. Muscle biopsies were obtained at rest and 3 and 6 h post exercise to examine amino acid transporter mRNA and protein expression.
RESULTS:
In both age groups, RE + EAA increased mRNA of L-type amino acid transporter 1 (LAT1)/solute linked carrier (SLC)7A5, sodium-coupled neutral amino acid transporter 2 (SNAT2)/SLC38A2, and cationic amino acid transporter 1/SLC7A1 (p < 0.05). SNAT2 protein increased in young at 3 and 6 h (p < 0.05), whereas old maintained higher LAT1 protein (p < 0.05). Compared to RE alone, RE + EAA enhanced amino acid transporter expression only in young (p < 0.05).
CONCLUSIONS:
RE increases muscle amino acid transporter expression in young and older adults, however, post exercise EAA ingestion enhances amino acid transporter expression only in young indicating that aging may influence the function of specific amino acid transporters.
Clin Nutr. 2012 Aug 1. Aging differentially affects human skeletal muscle amino acid transporter expression when essential amino acids are ingested after exercise.